Journal: Antibodies

Article Title: A Novel Panel of Rabbit Monoclonal Antibodies and Their Diverse Applications Including Inhibition of Clostridium perfringens Epsilon Toxin Oligomerization

doi: 10.3390/antib7040037

Figure Lengend Snippet: Summary of antibody function in different assays.

Article Snippet: Thermo Scientific Nunc MaxiSorp TM flat-bottom ELISA plates were incubated ON at 4 °C with 50 μL of the following amounts of active ETX: 0.3125 nM, 0.625 nM, 1.25 nM, 2.5 nM, 5 nM, 10 nM, 20 nM in 0.1 M coating buffer (Fisher Scientific Sodium Bicarbonate Crystalline Powder solubilized in DI H20).

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: Antibodies

Article Title: A Novel Panel of Rabbit Monoclonal Antibodies and Their Diverse Applications Including Inhibition of Clostridium perfringens Epsilon Toxin Oligomerization

doi: 10.3390/antib7040037

Figure Lengend Snippet: Detection of ETX via indirect ELISA. ( A ) ELISA plates were incubated with indicated amounts of ETX and then probed with 1 μg/mL of indicated anti-ETX monoclonal antibody. Displayed results are adjusted for background absorbance. ( B ) ELISA plates were coated with indicated amounts of pETX and probed with 1 μg/mL of the specified anti-ETX monoclonal. ( C ) Alternatively, ELISA plates were coated with 10 nM pETX and probed with the specified concentrations of anti-ETX antibodies. Wells probed with peroxidase-conjugated anti-rabbit antibody only (2AB Only) were used as negative controls. Results are mean ± SD.

Article Snippet: Thermo Scientific Nunc MaxiSorp TM flat-bottom ELISA plates were incubated ON at 4 °C with 50 μL of the following amounts of active ETX: 0.3125 nM, 0.625 nM, 1.25 nM, 2.5 nM, 5 nM, 10 nM, 20 nM in 0.1 M coating buffer (Fisher Scientific Sodium Bicarbonate Crystalline Powder solubilized in DI H20).

Techniques: Indirect ELISA, Enzyme-linked Immunosorbent Assay, Incubation

Journal: Antibodies

Article Title: A Novel Panel of Rabbit Monoclonal Antibodies and Their Diverse Applications Including Inhibition of Clostridium perfringens Epsilon Toxin Oligomerization

doi: 10.3390/antib7040037

Figure Lengend Snippet: Detection of pETX via Sandwich ELISA. ( A ) To test possible capture antibodies, JL001.2 with the rabbit Fc regions (JL001.2-Rb) and JL004 with the rabbit Fc region (JL004-Rb) were coated on ELISA plates and incubated with 0.6 nM and 7.5 nM pETX. JL001.2 with the mouse Fc region (JL001.2-Ms) was used as the detection antibody. ( B ) Sensitivity of pETX detection using indirect ELISA (JL001.2-Rb) was compared to the sensitivity of the sandwich ELISA using JL004-Rb as capture antibody and JL001.2-Ms as the detection body. The sandwich ELISA was more sensitive than the indirect ELISA method. The limit of detection (LOD) for the sandwich ELISA (red-dotted line) was 2.65 pM and 13.68 pM for the indirect ELISA (black dotted line). Results are mean ± SD.

Article Snippet: Thermo Scientific Nunc MaxiSorp TM flat-bottom ELISA plates were incubated ON at 4 °C with 50 μL of the following amounts of active ETX: 0.3125 nM, 0.625 nM, 1.25 nM, 2.5 nM, 5 nM, 10 nM, 20 nM in 0.1 M coating buffer (Fisher Scientific Sodium Bicarbonate Crystalline Powder solubilized in DI H20).

Techniques: Sandwich ELISA, Enzyme-linked Immunosorbent Assay, Incubation, Indirect ELISA